Fig. 7.

Ctr9/Paf1 subcomplex formation is essential for the integrity of a functional yeast Paf1C. a–d Ctr9/Paf1 subcomplex-mediated Paf1C assembly is essential for yeast cell viability. Strains of the indicated genotype were grown to late log phase/stationary phase (overnight) and plated in serial dilutions on a YPD plate. The plates are shown after 40 h of incubation at 30 °C (a), at 37 °C (b), on YPD containing 1 M NaCl (c), and on YPD containing 0.1 M hydroxyurea (HU) (d). e Representative immunoblot with specific H3K4me1/2/3 antibodies to detect methylation strength (top panels), or with H3 antibody to demonstrate equal loading (bottom panel). Bar graphs of H3K4me2 (f) and H3K4me3 levels (g) from various yeast strains, as indicated. All statistic data in this figure represents the results from three independent batches of experiments. Error bars represent s.e.m. (n = 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. Uncropped blots are shown in Supplementary Fig. 8