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. 2018 Sep 18;8:14003. doi: 10.1038/s41598-018-31849-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison between (left) synchrotron high-resolution X-ray microtomographic images (PRI mode, V_vox = 0.65³ μm³) of the left vocalis fibrous network and (right) 2D histological photomicrographs of the right vocalis excised from the same larynx L₁₀. (a) 2D coronal view of L₁₀-S₃, [C₂H₆0] = 70%. (b) Zoom on the banding patterns of the muscle fibres located within the yellow frame in (a). (c) 3D reconstruction of L₄-S₂, [C₂H₆0] = 30%. (d) 2D coronal view of L₁₀-S₅, prepared with Reticulin stain: Type III collagen fibres (black); muscular striated-cells or “fibres” (orange); adipocytes (white). (e) Zooms on the banding patterns of the muscle fibres located within the yellow frames in (d).