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. 2018 Sep 12;9:1304. doi: 10.3389/fpls.2018.01304

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Copyright © 2018 Biradar, Karan and Subudhi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cloning of SaSRP3-1 into E. coli expression vector pET101/D-TOPO^®. (A) Schematic representation of pET101/D-TOPO vector with cloning site, (B) Differences in the growth response curve of E. coli cells grown in the absence of NaCl in liquid LB culture. (C) Growth of E. coli cells after adding 600 mM NaCl to liquid LB media SaSRP3-1: pET-SaSRP3-1, ExCt1: pET-C. The mean of three independent measurements (OD₆₀₀) are indicated at different time intervals.