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. 2018 Sep 12;9:1304. doi: 10.3389/fpls.2018.01304

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Copyright © 2018 Biradar, Karan and Subudhi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plasmid construction for the gene SaSRP3-1 and development of transgenic plants. (A) The schematic diagram of the partial linear plasmid vector and position of the gene SaSRP3-1. The diagram depicts the details of the pCAMBIA1305.2 vector containing right and left borders, SaSRP3-1 gene between BglII and BstEII sites, 35S CaMV promoter, plant selection marker (hptII), multiple cloning sites, and termination sites; (B) Transformation and regeneration of Cocodrie-SaSRP3-1 transgenic rice plants showing selection, regeneration, and self-pollination of transgenic plants after Agrobacterium mediated transformation.