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. 2018 Sep 12;9:1304. doi: 10.3389/fpls.2018.01304

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Copyright © 2018 Biradar, Karan and Subudhi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirmation of SaSRP3-1 containing T₀ transgenic rice plants by PCR and detection of SaSRP3-1 mRNA by semi-quantitative RT-PCR analysis of transgenic seedlings grown in greenhouse condition. (A) PCR confirmation using SaSRP3-1 specific primers; (B) PCR confirmation of T₀ plants using hygromycin phosphotransferase (hptII) specific primers. M: 1 Kb DNA ladder, A1 to A24: DNA isolated from individual T₀ transgenic plants, PC: Positive control; (C) a representative RT-PCR experiment for detection of expression of elongation factor 1-α (ef1α) and SaSRP3-1 mRNA in seedling stage leaf samples of transgenic and Cocodrie (WT) plants. M: 1 Kb ladder, A7, A17, and A22: transgenic plants with SaSRP3-1 gene, NC: Negative control without any sample.