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Transgenic plants grown in green house and PCR analysis to confirm the integration of SaSRP3-1 and SaVHAc1 genes in pyramided progenies. (A) Pyramiding of SaSRP3-1 (T1) and SaVHAc1 (T2) genes by crossing homozygous plants during flowering stage; (B) Growth of pyramided progenies with both genes; (C) PCR amplification showing the presence of SaSRP3-1 gene; (D) Pyramided transgenic rice plants showing the amplification of SaVHAc1 gene using gene specific primers.
