FIG 3.
ZIKV-LAV has tumoricidal activity in glioblastoma bulk cells. (A) Immunofluorescence staining of glioblastoma bulk cells from 4121 GSC-implanted mice infected with ZIKV-LAV (MOI of 0.1). Bulk GBM cells were seeded in 24-well plates at a density of 1 × 105 per well and infected with ZIKV-LAV at an MOI of 0.1. Immunofluorescence staining was performed on day 3 postinfection. ZIKV E protein (green), Olig2 (red), and DAPI (blue) are shown. (B) Growth curves of ZIKV-LAV in bulk cells (MOI of 0.1). Viral RNA copies in culture fluids were analyzed by RT-qPCR at the indicated time points. (C) Bulk cells were seeded in 96-well plates at a density of 2,000 per well and infected with ZIKV-LAV at an MOI of 10. Cell viability was detected by the Cell Titer-Glo assay at the indicated time points postinfection. Statistical analysis was performed by two-way ANOVA. ****, P < 0.0001.