Figure 4.

miR-17-3p-Mediated Suppression of IR-Induced Three Mitochondrial Antioxidants

(A) miR-17-3p was induced in PC-3 cells and then treated with 6-Gy IR. The mRNA levels of MnSOD, Gpx2, and TrxR2 were quantified by qRT-PCR and then normalized by β-actin mRNA. (B) The protein levels of the three antioxidant enzymes were determined by western blots with β-actin normalization. (C) The cells were treated with different doses of IR as indicated, and the activities of the three antioxidant enzymes were measured. Mitochondria were isolated from the treated cells, and (D) the three antioxidant proteins and (E) their enzymatic activities were quantified corresponding to (B) and (C). Values are presented as the mean ± SD (n ≥ 3), and statistical significances between two groups are as described in Figure 1.