FIGURE 2.

Dnmt3a/3b knockout, even in conjunction with pharmacological Dnmt1 blockade, had no significant effect on mechanical hypersensitivity induced by nerve injury in mice. AdvCre^ERT2-Dnmt3a^fl/fl/Dnmt3b^fl/fl and their age-matched littermate controls (Dnmt3a^fl/fl/Dnmt3b^fl/fl, n = 9) were treated with tamoxifen and their mechanical hypersensitivity was assessed using von Frey filaments before (BL-1, BL-2) and after partial sciatic nerve ligation surgery (day 2, day 5, day 8, day 10, day 14). The Dnmt1 inhibitor RG108 was delivered intrathecally 30 min before surgery (day 0), as well as at varying intervals after surgery (day 1, day 2 and day 8, indicated by the black arrows). Plotted are 50% withdrawal thresholds in grams for the ipsilateral paw of each individual animal (diamonds for knockout mice, circles for their littermate controls), as well as their means and standard errors (red and gray bars for knockouts and littermates, respectively). The dotted line indicates the mean baseline withdrawal threshold. See Supplementary Table 1 for raw data and further details.