Table 1.
Number of transformants and number of strains with correct integrations obtained during transformation efficiency testing of the HSVtk fusion markers compared to their HSVtk-less (resistance gene only) controls.
HSVtk-nptII | nptII | HSVtk-nat1 | nat1 | HSVtk-hph | hph | |
---|---|---|---|---|---|---|
Number of candidates | 35 | 85 | 38 | 39 | 36 | 90 |
Targeted integration | 7 | 9 | 16 | 5 | 6 | 14 |
% Correct | 20.0% | 10.6% | 42.1% | 12.8% | 16.7% | 15.6% |
HSVtk-nptII and nptII: pPS45 and pPS45HL, integration locus: FGSG_02279; HSVtk-nat1 and nat1: pPS48 and pPS48HL, integration locus: FGSG_03278; HSVtk-hph and hph: pPS51 and pPS51HL, integration locus: FGSG_00348.