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. 2018 Sep 11;9:1954. doi: 10.3389/fmicb.2018.01954

Table 1.

Number of transformants and number of strains with correct integrations obtained during transformation efficiency testing of the HSVtk fusion markers compared to their HSVtk-less (resistance gene only) controls.

HSVtk-nptII nptII HSVtk-nat1 nat1 HSVtk-hph hph
Number of candidates 35 85 38 39 36 90
Targeted integration 7 9 16 5 6 14
% Correct 20.0% 10.6% 42.1% 12.8% 16.7% 15.6%

HSVtk-nptII and nptII: pPS45 and pPS45HL, integration locus: FGSG_02279; HSVtk-nat1 and nat1: pPS48 and pPS48HL, integration locus: FGSG_03278; HSVtk-hph and hph: pPS51 and pPS51HL, integration locus: FGSG_00348.