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. 2018 Sep 11;9:535. doi: 10.3389/fendo.2018.00535

Figure 5.

Figure 5

EV derived miRNAs can be used to distinguish between HaCaT and PKC EV sub-populations. The identified EV miRNAs were examined for correlations between the three EV populations derived from (A) HaCaT cells (n = 2 HaCaT p50 and p52); and (B) PKCs (n = 4 independent biological replicates) using Euclidean distance analysis, based on the total miRNA profile. The color scale indicates the degree of correlation between EVs, in which a dark blue color indicates greater correlation and lighter color scale indicates less correlation. Subsequently, hierarchical clustering of the 12 most statistically significant and differentially expressed miRNA from (C) HaCaT and (D) PKCs was performed and presented as a heatmap. The color scale indicates the abundance level of miRNAs, which were ranked based on p-values with the most significant first in the list. Hp50 and Hp52 indicate HaCaT cells were at passage 50 and 52; while 377, 366, 325, and 353 indicate skin donor numbers. AP, apoptotic bodies; MV, microvesicles; EX, exosomes. Heat maps were generated using R program (v 3.2.2).