Table 1.
Summary of small RNA sequencing from HaCaT samples.
| Biological repeats | HaCaT cells | AP | MV | EX | ||||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | |
| Total raw reads | 28,059,972 | 96,532,400 | 87,922,560 | 111,419,044 | 59,342,340 | 85,436,024 | 9,550,052 | 12,994,788 |
| Filtered reads | 6,136,227 | 18,013,320 | 21,125,475 | 25,768,444 | 14,295,262 | 19,905,748 | 2,017,355 | 2,244,521 |
| Number of alignments to Hg19 | 3,819,215 | 7,963,825 | 4,493,879 | 8,962,780 | 3,401,620 | 4,509,155 | 1,040,299 | 1,438,313 |
| Percentage of alignmentsa | 62.24% | 44.21% | 21.27% | 34.78% | 23.79% | 22.65% | 51.56% | 64.08% |
| Number of reads for identified miRNAs | 574,748 | 2,205,272 | 1,134,051 | 4,667,465 | 924,525 | 1,661,704 | 295,556 | 439,718 |
| Number of identified miRNAs (%b) | 861 (33%) | 1,107 (43%) | 929 (36%) | 1,217 (47%) | 879 (34%) | 1,022 (39%) | 727 (28%) | 799 (31%) |
| Number of miRNA with read = 1 | 233 | 230 | 183 | 240 | 226 | 223 | 194 | 205 |
a
Calculated as percentage of filtered reads.
b
Calculated as percentage of identified miRNAs to known mature miRNAs (miRBase release 21, 2,588 mature miRNAs).