Table 1.
Commercially available antibodies labeling zebrafish hair-cell synaptic proteins.
| Antigen | Structure Labeled | Host | Antibody type | Dilution | Company/Catalog # | Reference |
|---|---|---|---|---|---|---|
| Acetylated Tubulin | Afferents and hair cells | Mouse | Monoclonal IgG2b | 1:5000 | Sigma/T7451 | Harris et al., 2003; Murakami et al., 2003; Obholzer et al., 2008; Kindt et al., 2012 |
| Calretinin | Afferent processes | Mouse | Monoclonal IgG1 | 1:1000 | Swant/6B3 | Pei et al., 2016 |
| Choline Acetyltransferase | Cholinergic efferent terminals | Goat | Polyclonal | 1:500 | Millipore Sigma/AB144P | Zhang et al., 2018 |
| CtBP (1&2) | Synaptic ribbons | Mouse | Monoclonal IgG2a | 1:1000 | Santa Cruz/sc-55502 | Lv et al., 2016 |
| Cysteine String Protein | Synaptic vesicles | Rabbit | Polyclonal | 1:1000 | Millipore Sigma/AB1576 | Lin et al., 2016 |
| Gria 4 | AMPA glutamate receptor subunit | Rabbit | Polyclonal | 1:400 | Millipore Sigma/AB1508 | Sheets, 2017 |
| Grik 2 | Kainate glutamate receptor subunit | Rabbit | Polyclonal | 1:400 | Fitzgerald Industries/70R-1522 | Sheets, 2017 |
| Grik 4 | Kainate glutamate receptor subunit | Rabbit | Polyclonal | 1:400 | Genway Biotech Inc./GWB-DA6FF7 | Sheets, 2017 |
| Grin 1 | NMDA glutamate receptor subunit | Mouse | Monoclonal IgG2b | 1:1000 | Synaptic Systems/114011 | Sheets, 2017 |
| Human Natural Killer-1 | Afferent processes | Mouse | Monoclonal IgG1 | 1:500 | Developmental Studies Hybridoma Bank/ Zn-12 | Mo and Nicolson, 2011 |
| MAGUK | Postsynaptic densities | Mouse | Monoclonal IgG1 | 1:500 | NeuroMab (UC Davis)/ 75-029 | Sheets et al., 2011; Lv et al., 2016 |
| NSF | Hair cells and afferent process | Rabbit | Monoclonal | 1:50 | Cell Signaling/3924 | Mo and Nicolson, 2011 |
| Otoferlin | Hair cells | Mouse | Monoclonal IgG2a | 1:500 | Developmental Studies Hybridoma Bank/ HCS-1 | Faucherre et al., 2009; Goodyear et al., 2010 |
| Rab3 | Hair cells and efferent neurons | Mouse | Monoclonal IgG1 | 1:1000 | Synaptic Systems/107011 | Einhorn et al., 2012 |
| Synaptophysin 1 | Efferent terminals | Mouse | Monoclonal IgG1 | 1:1000 | Synaptic Systems/ 101 011 | Toro et al., 2015 |
| Tyrosine Hydroxylase | Dopaminergic efferents | Mouse | Monoclonal IgG2a | 1:1000 | Vector labs/ VP-T489 | Toro et al., 2015 |
| Vamp 2 | Efferent terminals | Rabbit | Polyclonal | 1:500 | Genetex/ GTX132130 | Zhang et al., 2018 |