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LPS-induced upregulation of the TLR4 protein level was repressed by fentanyl. BV-2 cells were either untreated, or treated with 5 µmol/l fentanyl, 1 µg/ml LPS or fentanyl followed by LPS. β-actin was used as the endogenous control. TLR4 protein levels were (A) measured by western blotting, then (B) quantified and statistically analyzed. **P<0.01 vs. control group; #P<0.05 vs. LPS group. Ctrl, control; TLR, toll-like receptor; LPS, lipopolysaccharide.
