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Effect of propofol on p53 and p21 expression in CRL-7566 cells. After 24 h of treatment with various concentrations of propofol (0, 1, 5 or 10 µg/ml), the (A) protein and (B and C) mRNA and expression levels of p53 and p21 in CRL-7566 cells were determined by western blot and reverse-transcription quantitative polymerase chain reaction analysis, respectively. Values are expressed as the mean ± standard deviation. *P<0.05, **P<0.01 vs. control group.
