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. 2018 Aug 10;16(4):3309–3316. doi: 10.3892/etm.2018.6594

Figure 2.

Figure 2.

TCONS_00024652 promotes the proliferation, migration and angiogenesis of HUVECs in vitro. (A) The relative expression of TCONS_00024652 in HUVECs following transfection with si-NC, si-TCONS_00024652-1 or si-TCONS_00024652-2 was measured using reverse transcription-quantitative polymerase chain reaction. (B) A Cell Counting Kit-8 assay was used 24 h following transfection with si-TCONS_00024652-1 and si-TCONS_00024652-2 to determine the proliferation of HUVECs. (C) The migratory ability of HUVECs was detected using a wound-healing assay following transfection with si-TCONS_00024652-1 (magnification, ×200). (D) Histogram presenting relative migration distance. (E) Transfected HUVECs were seeded onto Matrigel and then allowed to form capillary-like structures for 7 h (magnification, ×400). (F) Statistical analysis of the length of tubular structures in each group. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01 vs. control; ##P<0.01 vs. blank. Si, small interfering; HUVEC, human umbilical vein endothelial cells; TNF, tumor necrosis factor; OD, optical density.