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. 2018 Aug 10;16(4):3309–3316. doi: 10.3892/etm.2018.6594

Figure 3.

Figure 3.

miR-21 is a direct target of TCONS_00024652 in HUVECs. (A) Putative complementary sites within miR-21 and the 3′-untranslated region of TCONS_00024652 were predicted using starBase. (B) Relative expression of miR-21 in HUVECs transfected with si-TCONS_00024652. (C and D) Relative expression of TCONS_00024652 mRNA levels following transfection with miR-21 mimic or inhibitor, as determined using reverse transcription-quantitative polymerase chain reaction. (E) Luciferase activity in HUVECs following co-transfection with miR-21 and luciferase reporters containing WT- or mut-TCONS_00024652 transcript. Data are presented as the mean ± standard deviation. *P<0.05 vs. control. ##P<0.01 vs. blank. miR-21, microRNA-21; si, small interfering; NC, negative control; Mut, mutant; WT, wild-type; HUVEC, human umbilical vein endothelial cells.