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. 2018 Aug 10;16(4):3309–3316. doi: 10.3892/etm.2018.6594

Figure 4.

Figure 4.

miR-21 inhibitor reversed the suppression of HUVEC proliferation, migration and angiogenesis following TCONS_00024652 knockdown. (A) Relative expression of miR-21 in HUVECs following transfection with si-TCONS_00024652 and miR-21 inhibitor, as determined by reverse transcription-quantitative polymerase chain reaction (B) miR-21 inhibitor reversed the si-TCONS_00024652-induced inhibition of proliferation, as determined by a Cell Counting Kit-8 assay. (C) Wound healing assay was performed following transfection with si-TCONS_00024652 and miR-21 inhibitor and relative migration distance was calculated (magnification, ×200) (D) Transfected HUVECs were seeded onto Matrigel and incubated for 7 h; representative image and tube length analysis are presented (magnification, ×400). Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01 vs. control. #P<0.05 and ##P<0.01 vs. si-TCONS_00024652-1. Si, small interfering; miR-21, microRNA-21; HUVEC, human umbilical vein endothelial cells; OD, optical density.