Figure 3.

Deficiency of MCT4 promoted the expression of NKG2D and H60 in the tumor tissues. Tumor masses from mice inoculated with 4T1‐ShMCT4 “1” cells and 4T1‐Psuper.retro cells were harvested, and MCT4/NKG2D/H60 expression levels were determined. A, Representative images of tumor mass; B‐D, Quantitative, semiquantitative mRNA, or protein expression of MCT4/NKG2D/H60 by real‐time PCR, RT‐PCR, or Western blot analysis, respectively. E and F, MCT4/NKG2D/H60‐positive cells were stained by immunohistochemistry and immunofluorescence. Decreased MCT4 expression was accompanied by enhanced expression of NKG2D and H60, which are hallmarks of NK cytotoxicity, through modulating receptor‐ligand interaction of NK cells. * P < 0.05