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MCT4 deletion induced the compensatory upregulation of autophagy. A, The 4T1 cells treated with 7acc1 (0.1 mmol/L) and ShMCT4”1” 4T1 cell lysates were prepared for immunoblotting for lipidated LC3 and Beclin‐1. B, The 4T1 cells treated with 7acc1 (0.1 mmol/L) and ShMCT4”1” 4T1 cells were exposed to an agent, 3MA (2 mmol/L), that inhibited autophagy. The normalized impact of the agent on lysis was plotted. #P < 0.05. C, The schematic model of this study
