Figure 6.

FasL is essential for HCC cell growth suppression and apoptosis induction by NFATc1. NFATc1 overexpression plasmid and FasL siRNA were cotransfected into Huh7 and PLC cells. A, Relative OD values were examined by CCK‐8 assay at various time points (1, 2, 3, and 4 d) after transfection. OD value at day 1 was used for normalization. Dots represent data from cells in quadruplicate wells under the same treatment. B, Apoptosis rates were assessed by flow cytometry at 48 h post‐transfection. Dots represent data from cells in triplicate wells under the same treatment. Statistical analysis for all the experiment data in Figure 6 was performed using two‐tailed Student t tests. Data are presented as the mean ± SD. All experiments were performed in triplicate wells three times