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. 2018 Aug 7;7(9):4701–4717. doi: 10.1002/cam4.1716

Table 2.

PCR primer sequences used in this study

Target gene Gene ID Application Sequence (5′‐3′)
ACTB 60 Real‐time PCR F: GGACCTGACTGACTACCT
R: CTTAATGTCACGCACGATT
NFATC1 4772 Real‐time PCR F: TTCGGAATCAGAGGATAA
R: AGGCTCATAATCATCAGT
NFATC1 4772 RT‐PCR F: TAGCTCGAGCTATGCCAAGCACCAGCTTTCC
R: TATGGATCCCTGCTGTGGCAGCAGGGC
MAT1A 4143 CHIP‐qPCR F: AGACCAATCCAGATGAGAC
R: GTAGTGCTCCAGAGTTCA
FASLG 356 CHIP‐qPCR F: CACTGACCATTCTCCTGTAG
R: GCTATGCTCACCTTCCTG
MAT1A 4143 Real‐time PCR F: GAGAGTGCTTGTCCAGGTT
R: GAGAGGTTCCGTAGGTGAAG
MAT2A 4144 Real‐time PCR F: CAATCTACCACCTACAGCCAAGT
R: ACCGCCATAAGTGTCCACAAT
MAT2B 27430 Real‐time PCR F: GCAGTTCAGCAACAAGTC
R: GATCCAGCATTCTCTTCTCT
FASLG 356 Real‐time PCR F: GTTCTGGTTGCCTTGGTAG
R: CATCTGGCTGGTAGACTCT
FAS 355 Real‐time PCR F: GCCAATTCTGCCATAAGC
R: TTGTCTGTGTACTCCTTCC
BCL2L1 598 Real‐time PCR F: GTGCGTGGAAAGCGTAGA
R: CTAGGTGGTCATTCAGGTAAGTG
GPC3 2719 Real‐time PCR F: GGACAACGAGATAAGCACCTT
R: AAGCACACCACCGAGATG
CYCS 54205 Real‐time PCR F: GGAGGCAAGCACAAGACT
R: TTATTGGCGGCTGTGTAAGA
BCL2 596 Real‐time PCR F: TGTGTGGAGAGCGTCAAC
R: CGGTTCAGGTACTCAGTCAT
CASP8 841 Real‐time PCR F: GGAACAACTGGACAGTGAAG
R: CCTGGAGTCTCTGGAATAACA
CASP9 842 Real‐time PCR F: CGAACTAACAGGCAAGCA
R: AATCCTCCAGAACCAATGTC
VEGFA 7422 Real‐time PCR F: CTGCTCTACCTCCACCAT
R: ACCACTTCGTGATGATTCTG
KDR 3791 Real‐time PCR F: GGAGTCTGTGGCATCTGAAG
R: CGGTGGTGTCTGTGTCATC
BAX 581 Real‐time PCR F: CAAGAAGCTGAGCGAGTGT
R: GGCGGCAATCATCCTCTG
BAK1 578 Real‐time PCR F: CCGACGCTATGACTCAGAG
R: TGGTGGCAATCTTGGTGAA
DNMT1 1786 Real‐time PCR F: CGGCTTCAGCACCTCATT
R: AGGAACTCCACCACAATCTTG
FASLG promoter 356 RT‐PCR F: TATGGTACCCAGTTGGGCTCAGTGCAGT
R: CCGCTCGAGCTCTCGGAGTTCTGCCAGC