Figure 1.

MiR‐335‐5p had low expression in cisplatin‐resistant ovarian cancer cells A2780/DDP and could enhance their cisplatin sensitivity. A, Has‐miR‐335‐5p had a lower expression in A2780/DDP cells compared with A2780 cells detected by the microarray analysis; B, MTT assay showed IC₅₀ value of A2780/DDP cells was higher than A2780 cells, miR‐335‐5p could reduce IC₅₀ value of A2780/DDP cells and A2780 cells; C, MTT assay showed IC₅₀ value of A2780/DDP cells, and A2780 cells were increased after cells were transfected with miR‐335‐5p inhibitor; D, MTT assay showed that the cell viability of A2780/DDP cells was significantly reduced with miR‐335‐5p transfected, whereas A2780 cells were not significantly affected by miR‐335‐5p; E, It was found that miR‐335‐5p inhibitor suppressed the cell viability of A2780/DDP significantly but not A2780 cells; F‐H, Colony formation assay showed reduced colony number of A2780/DDP cells with miR‐335‐5p transfected, whereas A2780 cells were not affected by miR‐335‐5p. Besides, miR‐335‐5p inhibitor increased colony number of A2780/DDP significantly but not affected A2780 cells. *P < 0.05, **P < 0.01, compared with A2780/DDP group; ##P < 0.01, A2780 group compared with A2780/DDP group; DDP: cisplatin