| Reagent | Concentration |
| NaCl | 140 mM |
| KCl | 5 mM |
| MgCl2 | 1 mM |
| CaCl2 | 2 mM |
| HEPES (pH 7.4) | 10 mM |
| Glucose* | ~ 5 mM |
| *Use glucose to adjust osmolarity to 300–305 mOsm/L. |
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