Figure 4.

Effects of SAHA exposure on ING5 expression through miRNA expression downregulation in SH‐SY5Y cells. The target sites of miRNAs for ING5 mRNA were forecasted by miRNA software (A). SAHA treatment lowered miR‐640 (640), miR‐543 (543), miR‐624‐3p (624), and miR‐196‐b (196) expression in SY5Y cells as indicated by real‐time PCR (B). We successfully transfected mimics and inhibitors of the four miRNAs into SH‐SY5Y cells and found that SAHA exposure decreased miRNA expression (C). Western blot showed that both miR‐543 and miR‐196‐b overexpression decreased ING5 protein expression in SY5Y cells, but SAHA ameliorated this inhibition. The opposite effects were observed for their inhibitors (D). Note: S, SAHA treatment; mm, mimics; in, inhibitors. Each bar represents the mean ± standard deviation of three experiments, with the control as “1”. *P < .05 vs the control; #,P < .05 vs no SAHA treatment