Table 1. Antibodies for immunohistochemistry on human and mice paraffin sections.
| Antigen | Clone | Details* | Antigen retrieval** | Company | |
|---|---|---|---|---|---|
| Mice | EMR1 | F4/80 | rat, mono | PK | Bio-Lad/DAKO, Hercules, CA, USA |
| CD68 | FA11 | rat, mono | PK, TRS(H) | Bio-Lad/DAKO, Hercules, CA, USA | |
| CD163 | - | rabbit, poly | TRS (H) | CosmoBio, Tokyo, Japan | |
| CD204 | 2F8 | rat, mono | EDTA (H) | Bio-Lad/DAKO, Hercules, CA, USA | |
| CD206 | MR5D3 | rat, mono | PK | Bio-Lad/DAKO, Hercules, CA, USA | |
| Iba1 | - | rabbit, poly | EDTA (H) | WAKO, Tokyo, Japan | |
| Human | CD11b | EP1345Y | rabbit, mono | EDTA (H) | Abcam, Cambridge, UK |
| CD68 | PG-M1 | mouse, mono | EDTA (H) | Agilent Technol., Santa Vlara, CA, USA | |
| CD163 | 10D6 | mouse, mono | EDTA (H) | Leica Biosystems, Nussloch, Germany | |
| CD169 | Hsn7D2 | mouse, mono | EDTA (H) | Santa Cruz Biotech., Santa Cruz, CA, USA | |
| CD204 | SRA-E5 | mouse, mono | EDTA (H) | CosmoBio, Tokyo, Japan | |
| CD206 | 5C11 | mouse, mono | EDTA (H) | Abnova, Taipei, Taiwan | |
| Iba1 | - | rabbit, poly | EDTA (H) | WAKO, Tokyo, Japan |
*: mono; monoclonal antibody, poly; polyclonal antibody.
**: EDTA; 1mM EDTA (pH8.0), PK; proteinase K (5 min at RT), H; heat treatment, TRS; target retreival solution (DAKO).
EMR1; EGF-like module-containing mucin-like hormone receptor-like 1, Iba1;induction of brown adipocytes 1.