Table 2. Immunoreactivity of antibodies and antigen retreival methods.

		Antigen		Pretreatment (antigen retrieval methods)
				None		Protainase K		CB (M)		EDTA (M)		TRSN(M)		TRSD(M)		CB (P)		EDTA (P)		TRSN(P)		TRSD(P)
Mice		EMR1		+		+++		+		-		-		++		-		-		-		++
		CD68		+		++		-		-		-		+		-		-		-		+
		CD163		+		++		-		+		-		+++		-		+		-		+++
		CD204		-		+		-		-		-		-		-		++		+		-
		CD206		-		+++		-		-		-		+		-		-		-		++
		Iba1		++		+		++		+++		++		++		++		+++		++		++
Human		CD11b		+		+		++		+++		+++		+		++		+++		+++		++
		CD68		+		+++		++		++		++		++		++		+++		++		++
		CD163		-		-		++		++		++		++		++		+++		+++		++
		CD169		+		++		-		+++		+		+		-		+		-		-
		CD204		-		-		++		++		++		++		++		+++		+++		++
		CD206		++		-		++		++		++		++		++		+++		+++		++
		Iba1		++		+		+++		+++		+++		+++		+++		+++		+++		+++

-;negative, +; weakly positive, ++; moderately positive, +++; strongly positive.

(M); treatment with microwave, 5 min at 100^oC

(P); treatment with pressure cooker, 30 sec at 125^oC

CB; 10mM citrate buffer (pH6.0), EDTA; 1mM EDTA buffer (pH8.0), TRSN; target retrieval solution from NICHIREI (pH9.0), TRSD; target retrieval solution from DAKO (pH6.0).