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Allergic sensitization and challenge protocol. BALB/c mice were sensitized (day 1, 3, and 5) and challenged (day 12–14) intranasally with either PBS (30 μL/day; delivery vehicle; white triangles; n = 4), PM (33.3 μg/day, light grey triangles; n = 4), HDM (25 μg/day, dark grey triangles; n = 4) or HDM + PM (n = 4). Mice were euthanized 24 h after the final challenge (day 15) to assess pulmonary inflammation.
