Figure 4.

ZLc002 reduces co-immunoprecipitation of NOS1AP with nNOS but not PSD95-PDZ2 in HEK293T cells co-expressing the full-length proteins. ZLc002 (10 µM) treatment disrupts co-immunoprecipitation with full-length EGFP-nNOS of (a) full-length pLuc-NOS1AP but not of (b) pLuc-PSD95-PDZ2 from HEK293T cell lysates. Data are mean ± S.E.M. (n = 3) ***p < 0.001 (one-way ANOVA followed by Bonferroni’s post hoc test). Immunoblots under each bar chart demonstrate equal levels of nNOS among compared samples. GFP: green fluorescent protein.