FIGURE 3.

Effect of CD13 expression on drug resistance. PLC/PRF/5 cells were infected with lentivirus. After puromycin screening, PLC/PRF/5 cells with stable CD13 overexpression or knockdown were obtained (A). A representative immunoblot from three independent experiments giving similar results is shown for each western blot experiment. Densitometry for western blot was performed using AlphaEaseFC-v4.0.0 program. 1 × 10³ PLC/PRF/5 cells, vector control, stable CD13 overexpression or knockdown PLC/PRF/5 cells were seeded in 6-well plates. Approximately 1 week later, cells were dyed with 0.1% crystal violet, and then photographs were taken (B). The inhibition rate of different cytotoxic agents on overexpressed or knocked down cells were determined (C). Data represent mean ± SD (n = 3). ^∗P < 0.05.