Figure 4.

miR-99b decreases gastric cancer cell proliferation by suppressing autophagy. BGC-823 cells were pretreated with miR-99b mimics or negative control, and either left untreated or pre-treated with the autophagy inhibitor 3-MA, followed by exposing to H. pylori for 12, 24 and 48 h. (A) Intracellular bacteria loads at different time points. (B) Cell numbers at different time points as determined using an MTT assay. *P<0.05 vs. NC. miR, microRNA; 3-MA, 3-methyladenine; NC, negative control.