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Stability of Psy-Lig mutants measured by DSF. In the case that multiple transitions were observed, Tm values are given for the higher temperature, with the exception of K25A which had a single low Tm. (A) Difference in Tm with additives; reference condition is 0.1 mM ATP, 10 mM MgCl2. Asterisks mark conditions where no high-temperature transition was observed. (B) Tm of mutants in buffer C (50 mM Tris pH 8.0, 100 mM NaCl, 1 mM DTT, 5% glycerol) without any additives.
