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Binding affinity of Psy-Lig mutants to nicked DNA measured by MST. Values are the average of four replicates, error is given as standard deviation of the mean
| Psy-Lig mutant | EC50 (μM) |
|---|---|
| WT | 0.98 (± 0.042) |
| K25A | 3.30 (± 0.110) |
| R69A | 3.08 (± 0.142) |
| R80A | 3.62 (± 0.34) |
| N144A | 2.45 (± 0.10) |
| K176A | 5.17 (± 1.55) |
| K191A | ND* |
| K193A | 2.55 (± 0.110) |
| K212A | 7.10 (± 0.076) |
| S215A | 1.74 (± 0.087) |
| K191A/K212A | ND* |
| OB-Ala | ND* |
*ND = not determined.
