Figure 2.

C>G-less genomic regions are devoid of significant Rho-dependent signals. (A) Schematic depiction of the DNA templates used in our standard in vitro transcription termination experiments. (B) Representative denaturing PAGE gels illustrate the absence of formation of Rho-specific truncated transcripts during transcription of C>G-less templates. The RO bands correspond to runoff transcripts. RNAs migrating more slowly than the RO bands result from template switching events (once RNAP has reached a template end; see (12) and references therein). (C) Representative transcription experiments with the four unusual C>G-less templates yielding low amounts of truncated transcripts (identified by stars next to the gels) in the presence of Rho.