Figure 3.

Rho-dependent termination signals encoded by DNA templates containing C>G bubbles. Representative denaturing PAGE gels illustrate the different classes of transcription termination signals. Rho did not change the transcription profiles for 5.6% of the tested ‘C>G-plus’ DNA templates (‘None’ category). Rho-dependent signals were considered ‘Weak’ when bands migrating faster than runoff transcripts appeared in the presence of Rho while the intensity of the ‘runoff’ band was hardly affected (47.2% of the ‘C>G-plus’ templates). They were considered ‘Strong’, when Rho elicited the appearance of fast-migrating bands and a sharp decrease of the intensity of the runoff band (47.2% of the ‘C>G-plus’ templates).