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. 2018 Jun 5;46(16):8605–8615. doi: 10.1093/nar/gky488

Figure 2.

Figure 2.

Bacillus subtilis YqfG is essential and is necessary for 3′ processing of 16S rRNA. (A) Schematic of 16S rRNA and precursor sequences. The mature 16S rRNA sequence is in green and precursor sequences in black. Processing reactions are shown in red. (B) Sequence alignment of Escherichia coli YbeY and B. subtilis YqfG. Identical amino acids are indicated by an asterisk; strongly similar amino acids by a colon; weakly similar amino acids by a full stop. Mutations were made in amino acids labelled in red (see below). (C) Spot dilution assays (log scale) of wt strain and CCB751 (Pspac-yqfG + pMAP65) in the presence and absence of IPTG. Mid-log phase cells were diluted to the same OD, then diluted in a 10-fold dilution series and 2 μl was spotted on plates. Note that the CCB751 strain was grown in the presence of antibiotics MLS and kanamycin to maintain the Campbell integrative and replicative plasmids. (D) Depletion of YqfG results in a lack of 16S 3′ processing and release of the 65 nt downstream fragment. Northern blot probed with an oligo (CC172) against the 3′ extension.