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. 2018 Jul 24;46(16):8532–8541. doi: 10.1093/nar/gky663

Figure 3.

Figure 3.

(A) Illustration of a two-level cascading circuit YES gate. The first-level YES gate consists of the duplex complex D, single strand C’, catalyst H4 and input DNAzyme-3. The second-level YES gate is composed of the duplex complex B*, single strand A, fluorescent reporter BrA and catalyst H1. The output DNAzyme-2a in the first-level gate triggers the downstream reaction as an input to induce a significant fluorescence increase. (B) Illustration of three DNAzyme catalytic reactions (DNAzyme-3, DNAzyme-2a and DNAzyme-1) and two entropy-driven reactions (catalysts H4 and H1) in the two-level cascading circuit. (C) Time-dependent fluorescence changes with different inputs. The time interval is 6 min. Curves (1), (2) and (3) demonstrate the effect of the addition of (1) DNAzyme-3 without catalysts H1 and H4, (2) with catalysts H1 and H4 in the absence of DNAzyme-3, (3) in the presence of DNAzyme-3 and both catalysts, respectively. All data represent the average of three replicates. Error bars represent one standard deviation from triplicate analyses.