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. 2018 Jul 24;46(16):8532–8541. doi: 10.1093/nar/gky663

Figure 5.

Figure 5.

(A) Illustration of a self-catalysis circuit. With the help of catalyst H1, DNAzyme-2 is used as an input to trigger the whole circuit, generating two products: DNAzyme-2b and DNAzyme-4. DNAzyme-2b has the same enzyme cleavage site as the input DNAzyme-2 and can therefore cut strand B*-CrD to initiate the self-catalysis reaction. (B) Illustration of a control experiment. DNAzyme-2 is used as an input to trigger the whole circuit, generating two products: DNAzyme-5 and DNAzyme-4. DNAzyme-5 is a waste product. (C) Abstract graph of three DNAzyme catalytic reactions (DNAzyme-2, DNAzyme-2b and DNAzyme-4) and one entropy-driven reaction (catalyst H1) in the self-catalysis circuit and control experiment. (D) Analysis of the self-catalysis circuit products using a 12% PAGE gel. Lanes 1, products of the duplex substrate B*; lane 2, the product of DNAzyme-4; lanes 3–6, a series of different DNAzyme-2 concentrations added to the gate solution with strands A0 and A2 as 0.05, 0.15, 0.45 and 1.35 μM, respectively; lanes 7–10, a series of different DNAzyme-2 concentrations added to the gate solution with strands A1 and A2 as 0.05, 0.15, 0.45 and 1.35 μM, respectively. Other DNA components were used at fixed concentrations 0.6 μM of B’/B*-CrD, 0.15 μM of catalyst H1 and 0.1 μM of ssDNA (A0, A1 and A2). (E) Fluorescence signal analysis. The columns 1–6 correspond to the relative fluorescence increase percentage (△F) before and after self-catalyzing at 0.1, 0.2, 0.4, 0.6, 0.8 and 1.0 μM, respectively.