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. 2018 Sep 12;9:2192. doi: 10.3389/fmicb.2018.02192

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Copyright © 2018 Chiba, Hill-Batorski, Neumann and Kawaoka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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UAP56 interacts with NS1 proteins derived from different influenza A viruses. HEK293T cells were transfected with plasmids expressing FLAG-tagged UAP56 proteins and/or the NS1 proteins of A/Brevig Mission/1/1918 (H1N1; BM/1/1918) (A), A/Vietnam/1203/2004 (H5N1; VN1203) (B), or A/Anhui/1/2013 (H7N9; AH1) (C). At 48 h post-transfection, the cells were lyzed and immunoprecipitated with anti-FLAG M2 antibody-conjugated magnetic beads. Co-precipitated proteins were analyzed by immunoblotting with anti-NS1 antibody.