Figure 4.

Overexpression of CCND2 partially rescues the inhibitory effects of miR-409-3p on cell proliferation and cell cycle progression. TPC-1 and GLAG-66 cells were transfected with mimic NC or miR-409-3p mimics together with a plasmid expressing CCND2 or an empty vector. (A) CCK-8 assays were performed to determine the proliferation rate of TPC-1 and GLAG-66 cells after 48 h transfection. Cell cycle analysis was performed 48 h after transfection in (B) TPC-1 and (C) GLAG-66 cells. Values represent the mean from three separate experiments; error bars represent the standard deviation. *P<0.05 vs. NC and ^#P<0.05 vs. miR-409-3p mimics. CCND2, gene encoding cyclin D2; miR-409-3p, microRNA-409-3p; NC, negative control.