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. 2018 Aug 8;16(4):5299–5308. doi: 10.3892/ol.2018.9281

Figure 2.

Figure 2.

OPN induced the expression of Twist through the PI3K/Akt signaling pathway. (A) Western blot analysis demonstrates the effect of overexpression of OPN on the protein levels PI3K and Akt in HCC cells. (B) Western blot analysis depicting the effect of knockdown of OPN on the protein levels of PI3K and Akt in HCC cells. (C) Suppression of Akt activation by the specific PI3K/Akt inhibitor LY294002 markedly attenuated OPN-elicited EMT. OPN, osteopontin; PI3K, phosphoinositide 3-kinase; Akt, RAC serine/threonine-protein kinase; HCC, hepatocellular carcinoma; EMT, epithelial-mesenchymal transition; NC, negative control; uPA, urokinase; MMP2, matrix metalloproteinase 2; p-Akt, phosphorylated Akt; shOPN, short hairpin RNA targeted at OPN.