Table I.
Dataset | Sample type | Assay type | miRNA probes, n | Method of validation |
---|---|---|---|---|
SY (23) | Three human breast tumors | Multiplex Real-time PCR Assay (Applied Biosystems; Thermo Fisher Scientific, Inc.) | b- | Breast cancer cell colony formation assay |
IM (11) | 10 normal and 76 neoplastic breast tissues | miRNA microarray chip (KCI version 1.0) and Perkin-Elmer ScanArray XL5K | 368 | Northern blotting |
HH (25) | 148 patients with BC and 44 age-matched and disease-free controls | TaqMan (Applied Biosystems; Thermo Fisher Scientific, Inc.) | – | RT-qPCR |
LA (26) | – | – | – | – |
KL (27) | MCF-7 and MDA-MB-231 cell lines | RT2 miRNA PCR array system (SABiosciences™ Corporation, Frederick, MD, USA) | 376 | RT-qPCR |
YL (28) | 113 BC and 40 paired non-cancerous NATs | CapitalBio (http://www.capitalbio.com) | 509 | RT-qPCR |
SH (29) | 48 tissue and 100 serum samples of patients with primary BC and 20 control samples | – | 10 | RT-qPCR |
VS (40) | 63 primary tumors and 177 normal tissues | Bead-based microRNA profiling platform | 40-mer | Northern blotting |
BC, breast cancer; RT-qPCR, reverse transcription quantitative polymerase chain reaction; miRNA, microRNA; NATs, normal adjacent tumor tissues; -, data not available from the orignal datasets.