Figure 3.

INH and INH+NAC effects on in vitro granulomas developed using immune cells from healthy subjects. Survival of M. tb inside INH and INH+NAC-treated granulomas (A), Hematoxylin and Eosin staining of INH and INH+NAC-treated granulomas from healthy individuals (B), determination of GSH levels at 15 days post-infection (C), IFN-γ levels at 15 days post-infection (D), IL-6 levels at 15 days post-infection (E), TNF-α levels at 8 days (F) and 15 days (G) post-infection, and phagosome acidification at 15 days post-infection (H,I). INH+NAC treatment resulted in a significant and complete clearance of M. tb infection inside the granulomas from healthy subjects compared to treatment with INH alone (A). INH+NAC treatment also induced formation of solid and stable granulomas (B). ^*p < 0.05 when comparing samples treated with INH only to those treated with INH and NAC. ^#p < 0.05 when comparing samples treated with INH and NAC to their controls.