Table 1.
Foraminifer sampling locations, ecology, and sample preparation for nitrogen isotopic analysis of amino acids
| Species | Sample ID | Sampling site | Condition | Sampling date | Type of symbiosisa | Origin of symbiontb | Test typec | Microhabitat in coralline algaed | Mode of lifee | H2O2 treatmentf | Analyzed partg | Number of individual specimens |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P. opercularis | 160108‐3 | Omaezaki, Shizuoka | Nature (Day) | 21 April 2015 | K | B | Hyaline, High Mg | F | C | − | Cell | 200 |
| 111205‐4 | Minami‐izu, Shizuoka | Nature (Day) | 13 July 2011 | − | Cell | 25 | ||||||
| 120607‐1 | Yugawara, Kanagawa | Nature (Day) | 8 May 2012 | + | Test | 122 | ||||||
| 120607‐2 | − | Cell | 122 | |||||||||
| 160118‐2 | − | Cell | 35 | |||||||||
| G. patelliformis | 120606‐7 | Yugawara, Kanagawa | Nature (Day) | 8 May 2012 | K | B | Hyaline, High Mg | F | C | + | Test | 69 |
| A. quadrangularis | 120607‐3 | Yugawara, Kanagawa | Nature (Day) | 8 May 2012 | K | B | Hyaline, High Mg | F | C | + | Test | 80 |
| 120607‐4 | − | Cell | 79 | |||||||||
| 160112‐2 | 20 April 2015 | − | Cell | 42 | ||||||||
| 160118‐3 | Nature (Night) | 19 April 2015 | − | Cell | 88 | |||||||
| E. crispum | 120606‐1 | Yugawara, Kanagawa | Nature (Day) | 8 May 2012 | K | B | Hyaline, High Mg | F | P | + | Test | 131 |
| 120606‐2 | − | Cell | 131 | |||||||||
| 160112‐3 | 20 April 2015 | − | Cell | 100 | ||||||||
| 160118‐4 | Nature (Night) | 19 April 2015 | − | Cell | 100 | |||||||
| P. nipponica | 160108‐4 | Omaezaki, Shizuoka | Nature (Day) | 21 April 2015 | * | B | Hyaline, Low Mg | F | P | − | Cell | 200 |
| 120606‐5 | Yugawara, Kanagawa | Nature (Day) | 8 May 2012 | + | Test | 90 | ||||||
| 120606‐6 | − | Cell | 90 | |||||||||
| C. lobatulus | 160112‐1 | Omaezaki, Shizuoka | Nature (Day) | 21 April 2015 | None | ‐ | Hyaline, Low Mg | F, S, T | A | − | Cell | 95 |
| 120606‐3 | Yugawara, Kanagawa | Nature (Day) | 8 May 2012 | + | Test | 99 | ||||||
| 120606‐4 | − | Cell | 98 | |||||||||
| 160112‐4 | 20 April 2015 | − | Cell | 58 | ||||||||
| 160126‐1 | Nature (Night) | 19 April 2015 | − | Cell | 60 | |||||||
| Q. yabei | 120607‐5 | Yugawara, Kanagawa | Nature (Day) | 8 May 2012 | None | ‐ | Porcellaneous, High Mg | S, T, Fl | F | + | Test | 11 |
| 120607‐6 | − | Cell | 10 | |||||||||
| 160118‐1 | 20 April 2015 | − | Cell | 27 | ||||||||
| 160126‐2 | Nature (Night) | 19 April 2015 | − | Cell | 42 |
Shading indicates sampling site and period; white: collected from Omaezaki or Minami‐Izu in the daytime, gray: collected from Yugawara in the daytime, dark gray: collected from Yugawara at night.
aK, kleptoplast;bB, Bacillariophyta (diatom). cHigh Mg, high magnesian calcite; low Mg, low magnesian calcite (from Toyofuku, Kitazato, Kawahata, Tsuchiya, & Nohara, 2000; Toyofuku et al., 2011). dF, frond; S, stem; T, thallus; Fl, flocculent layer. Classification of foraminiferal microhabitats in coralline algae from Kitazato (1988). eC, crawling form; P, phytal form; A, attached form; F, free living. Rocky‐shore foraminiferal modes of life were from Kitazato (1988). f+, with H2O2 treatment—measures the nitrogen isotopic composition of organic membranes within the calcareous shell (intracrystalline protein); −, without H2O2 treatment—measures the nitrogen isotopic composition of both cytoplasm and intracrystalline proteins. gTest, organic matters including both organic sheet and intracrystalline protein in the test; cell, organic matter in the test and cytoplasm.
*Endobiotic algae.