Figure 6.

Analysis of immune gene expression and cytology of rCXCL8a- and rCXCL8b-elicited tadpole cells. Tadpoles and adult frogs were injected intraperitoneally with rCXCL8a or rCXCL8b (1 μg/g of body weight) in 10 μl of saline or with an equal volume of the vector control (supernatants from empty vector-transfected Sf9II cell, processed in parallel to rCXCL8a and rCXCL8b production). After 4 h, animals were lavaged with saline and the cells were enumerated (A). The tadpole (B,C) and adult frog (not presented) rCXCL8a and rCXCL8b-elicited cell immune gene expression was examined relative to the Gapdh control (N = 6). All results are presented as means + SEM. Above-head letters denote statistical designations: experimental groups described by distinct letters are statistically different (P < 0.05) while those marked by the same letters are not. (D) Tadpole rCXCL8a and rCXCL8b-elicited cells were stained with a rabbit anti-frog rG-CSFR (or saline) Ab and secondary goat anti-rabbit Ab and examined by confocal microscopy.