Average weighed mouse is 20 g

∴ 8.575 mg → 20 g

x → 1000 g

$x=\frac{1000\times 8.575}{20}=428.75\text{mg}/\text{kg}={\text{IMLD}}_{50}$

But the ED50 reported by Aguilar et al. [1] is 477 mg/kg

But IMLD50 = 451.3 mg/kg

∴ ${\text{LD}}_{50}\text{of the venom}=\frac{{\text{ED}}_{50}}{3}\times \text{Wm}\times {10}^{-4}=\frac{{\text{IMLD}}_{50}}{3}\times \text{Wm}\times {10}^{-4}$

$=\frac{428.75}{3}\times 20\times {10}^{-4}$

LD50 = 0.29 mg/kg

IMLD50 ≃ ED50

∴ IMLD50 can be used to calculate ED50