Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer sequences used for quantitative polymerase chain reaction analysis.
| Gene | Primer sequence (5′-3′) |
|---|---|
| IR | F: TCATGGATGGAGGCTATCTGGA |
| R: TCCTTGAGCAGGTTGACGATTTC | |
| IRS-1 | F: AAGCACCTATGCCAGCATCAAC |
| R: GAGGATTGCTGAGGTCATTTAGGTC | |
| PI3K | F: CCAGAAGAAGGGACAGTGGTATG |
| R: TCGTAGCCAATCAGGGAGGT | |
| AKT | F: ATGGACTTCCGGTCAGGTTCA |
| R: GCCCTTGCCCAGTAGCTTCA | |
| GAPDH | F: GGCACAGTCAAGGCTGAGAATG |
| R: ATGGTGGTGAAGACGCCAGTA |
IR, insulin receptor; IRS-1, IR substrate-1; PI3K, phosphoinositide 3-kinase; AKT, protein kinase B.
