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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Immunol. 2018 Aug 10;201(7):2004–2015. doi: 10.4049/jimmunol.1800070

Figure 5.

Figure 5.

IL-10 facilitates alternative activation of macrophages in lungs of C. neoformans-infected mice. Lung-derived single cell suspensions harvested 6 wpi from IL-10+/+ or IL-10−/− mice were stained and analyzed by flow cytometry to identify alveolar and exudate macrophages. (A and B) Cell surface expression of CD40, CD80 and CD86 on alveolar (A) and exudate (B) macrophages was assessed by determining the ∆ geometric mean fluorescence intensity (GMFI) of each sample relative to an isotype-matched control antibody-stained sample. (C and D) The percentage of cells expressing iNOS, arginase 1 and CD206 in the indicated macrophage subpopulations was assessed using intracellular staining. (A-D) Bar graph data are presented as mean ± SEM of 4 or 5 mice per group. IL-10+/+ mice, black bars; IL-10−/− mice, white bars. * P < 0.05, *** P < 0.001; t test. Similar results were obtained in 2 independent experiments. MQ, macrophages; Arg 1, arginase 1.