Skip to main content
. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Immunol. 2018 Aug 10;201(7):2004–2015. doi: 10.4049/jimmunol.1800070

Figure 6.

Figure 6.

BM-DCs pulsed with cryptococcal MPs up-regulate expression of IL-10 and IL-10 receptor, which promotes DC type-2 activation. BM-DCs generated from IL-10+/+ (A-L) and IL-10−/− (C-L) mice were pulsed with C. neoformans-MPs, LPS or PBS (vehicle) for 24 hours. (A, C, E, G-I and K-L) Transcript levels of IL-10 (A), IL-10R1 (C), CD40 (E), iNOS (G), IL-12A (H), IL-12B (I), Arginase 1 (K) and Mrc1 (CD206, L) in pulsed BM-DCs were quantified by qRT-PCR analysis. Expression level was normalized to GAPDH and expressed relative to the mean expression of PBS-pulsed IL-10+/+ DCs. (B and J) Concentration of IL-10 (B) and IL-12 (J) in supernatants of pulsed BM-DCs was quantified using a cytometric bead array. (A-C, E, G-L) Data are reported as mean ± SEM of triplicates. ** P < 0.01, *** P< 0.001; one-way ANOVA followed by Tukey’s test for multiple comparisons (A and B), t test corrected for multiple comparisons using the Holm-Sidak method for comparing IL-10−/− to IL-10+/+ using the same pulsing condition or two-way ANOVA followed by Tukey’s test for multiple comparisons for comparing IL-10+/+ DCs pulsed with C. neoformans-MPs or LPS versus PBS. Similar results were obtained in 2 independent experiments. IL-10+/+ BM-DC, black bars; IL-10−/− BM-DC, white bars. (D and F) Pulsed BM-DCs were stained using a fluorochrome-conjugated antibody targeting the IL-10 receptor (D) or CD40 (F) and analyzed by flow cytometry. Histogram overlays show cell surface expression of the IL-10 receptor (D) or CD40 (F). Control antibody staining, black histogram; target antibody staining of IL-10+/+ cells, thick line clear histogram; target antibody staining of IL-10−/− cells, thin line shaded histogram. MP, Mannoprotein ; IL-10R, IL-10 receptor.