. Author manuscript; available in PMC: 2018 Sep 19.

Published in final edited form as: Methods Cell Biol. 2016 Feb 28;133:229–252. doi: 10.1016/bs.mcb.2015.12.002

Table 1.

Parameters for Three Zebrafish Hair-Cell Calcium Imaging Systems

	Nikon Widefield	Bruker Sweptfield Confocal	Nikon C2 Line- Scanning Confocal
Detector	Camera - Hamamatsu	Camera - Rolera	PMT - Nikon
	ORCA-R2	EM-C²	Nikon C2
	Gain 50/100	EM gain 3700/4000	Gain 75/255
Bin	2 × 2	2 × 2	−
ROI	50 × 50 μm	50 × 50 μm	50 × 50 μm
X-Y resolution	0.43 μm/pixel	0.53 μm/pixel	0.33 μm/pixel
Slit size	−	35 μm slit	−
Light source power	Intensilight (mercury) 25% with cy3 filter	Solid state 561 nm laser 5%	Solid state 561 nm laser 5%
Speed	10 fps	10 fps	7.5 fps
Optical section	Largest, worst	Variable depending on pinhole or slit size	Thinnest, best
Max speed	100 fps	100 fps	10 fps
Main advantages	Inexpensive	Fast, high optical resolution	Highest optical resolution
Main disadvantages	Low optical resolution	Cost	Slow speed, cost, photobleaching